Abstract
Three bioassay methods with 2 aquatic arthropods, Gammarus pseudolimnaeus (Crustacea: Amphipoda) and Aedes aegypti larvae (Insecta: Diptera), in artificial aquatic habitats were used to assess the acute lethal effects of permethrin due to downwind drift deposits from mistblower applications. In 3 separate tests, all methods produced similar results, namely, that single line sprays of permethrin at 35 g Al/ha resulted in mortality only within 30 m of the insecticide source. An application of 17.5 g AI/ha produced proportionately lower mortality. Mortality of amphipods was generally higher than that of mosquito larvae. The bioassay methods were very sensitive. Standards conducted concurrently with the tests gave LC 50 values of 0.25–0.37 ppb for G. pseudolimnaeus and 0.69–1.85 ppb for A. aegypti larvae.
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