Abstract
This study examines the use of a covalently selenium-bonded peptide and phage that binds to the Yersinia pestis F1 antigen for the targeting and killing of E. coli expressing this surface antigen. Using a Ph.D.-12 phage-display library for affinity selection of the phage which would bind the F1 antigen of Y. pestis, a phage displaying a peptide that binds the F1 antigen with high affinity and specificity was identified. Selenium was then covalently attached to the display phage and the corresponding F1-antigen-binding peptide. Both the phage and peptides with selenium covalently attached retained their binding specificity for the Y. pestis F1 antigen. The phage or peptide not labeled with selenium did not kill the targeted bacteria, while the phage or peptide labeled with selenium did. In addition, the seleno-peptide, expressing the F1 targeting sequence only, killed cells expressing the F1 antigen but not the parent strain that did not express the F1 antigen. Specifically, the seleno-peptide could kill eight logs of bacteria in less than two hours at a 10-µM concentration. These results demonstrate a novel approach for the development of an antibacterial agent that can target a specific bacterial pathogen for destruction through the use of covalently attached selenium and will not affect other bacteria.
Highlights
Antibiotic resistance is rising to dangerously high levels in all parts of the world
We investigated our hypothesis by testing the ability of the seleno-phage to selectively kill E. coli expressing the Y. pestis F1 antigen XL1-blue/pYPR1)
We have shown that a selenium-conjugated display phage and a Se-conjugated peptide that binds the F1 antigen of Y. pestis can kill E. coli expressing this antigen while sparing those that do not
Summary
Antibiotic resistance is rising to dangerously high levels in all parts of the world. The presence of added oxygen with seleno-peptide #8 killed over 90% of the bacteria expressing the Y. pestis F1 antigen in 1 h and most bacteria in 2 h at a 10 μM concentration. C. ocloili(X(XLL1-1b-blulue)e)isisththeepparaernent tstsrtarianinwwitihtohuout thteheplpalsamsmididfofrorthteheF1F1anatnigtiegne.n. It is important to note that the E. coli (XL1-blue) parent strain, without the plasmid for the F1 antigen, shows no killing by peptide-8 with selenium. It is important to note that the E. coli (XL1-blue) parent strain, without the plasmid for the F1 antigen, shows no killing by peptide-8 with selenium. Since the selenopeptide could only kill bacteria expressing the targeted protein, it shows the selectivity of this type of antimicrobial
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
