Abstract
This report covers practical aspects of boiling a mass of tissue in a liquid amine: description of the necessary apparatus, consideration of important factors as temperature and water dilution, and a brief account on the recommended agents. Boiling is accomplished in a thermal shock-resistant glass vial connected with a larger bottle which serves as an expansion chamber. Both are containers in common use for injectibles. Connection is made by a double-pointed hypodermic needle which perforates both rubber stoppers. A 40 w electric bulb supplies the heat. The specimen is boiled in a volume of amine 5 times that of the tissue mass. The liquid must be discarded after a single use. Ethylenediamine and 1, 3-propanediamine are the most effective or rapid-acting agents. Hydroxyalkyl derivatives are regarded as slow-acting, but their use has advantages due to softening of the basal membranes. Intermediate results are obtained by the use of mixtures of amines.
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