Abstract
THE USE OF ALKALINE EARTH METAL PICRATES FOR THE ISOLATION OF l-PHENYLALANINE FROM PROTEIN HYDROLYSATES
Highlights
The method described consists of preparing, from protein hydrolysates, a fraction containing most of the phenylalanine present but substantially free from the other amino acids which form sparingly soluble derivatives with strontium picrate
The pure amino acid was obtained from egg albumin hydrolysates in amounts corresponding to 5.1 per cent of the dry and ash-free protein, representing a recovery of 72 per cent of the amount present according to the analysis of Tristram [12]
Use has been made of the finding that phenylalanine forms sparingly soluble complex salts with t,he alkaline earth metal picrates for the isolation of this amino acid from protein hydrolysates
Summary
The method described consists of preparing, from protein hydrolysates, a fraction containing most of the phenylalanine present but substantially free from the other amino acids which form sparingly soluble derivatives with strontium picrate. Attempts to use this reagent for the preparation of phenylalanine from egg albumin hydrolysatcs resulted in low yields This has been shown to be due to the tendency of the phenylalanine-barium picrate complex to undergo decomposition, in aqueous solutions, into its two components. The hydrolysate, SELIM, RAMADAN, AND EL-SADR freed from inorganic ions, was concentrated in vacua to about 2 liters and saturated with H& To this solution picric acid (22 gm.) was added and dissolved by stirring. Recrystallization of the ethyl acetate-treated product from hot water resulted in partial decomposition of the complex with the formation of additional barium picrate. The yield of pure L-phenylalanine isolated from 200 gm. of egg albumin was 5.3 gm
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
