The Use of Agricultural Waste to Increase the Production Ligninolytic Enzyme by Fungus <i>Polyporus</i> sp.

Abstract

This research was aimed at enhancing the production of ligninolytic enzymes produced by fungus Polyporus sp. isolated from Buleleng as a means to treat the textile waste water. The optimum enzyme production was carried out by adding agricultural waste such as banana skin, straw, and sawdust. The research was completed in several stages including i) growing the fungus Polyporus sp. in PDA media, ii) enzymes production, iii) enzyme activity tests, and iv) decomposition analysis of textile waste water in several enzyme concentrations and in various incubation time lengths. The results showed that the optimum activities of laccase, MnP, and LiP enzymes that were produced without the addition of any agricultural waste were 20.5, 25.7, and 75.4 μmol/mL·minute. On the other hand, the enzymes activities on the addition of banana skins were 139.0, 116.0, and 654.0 μmol/mL·minute, on the addition of sawdust were 194.4, 41.0, and 259.0, and on the addition of straw were 148.2, 131.0, and 392.0 μmol/mL·minute, respectively. The efficiency of the degradation of 25 mL of remazol black B and remazol red RB (100 mg/L) by using 6 mL ligninolytic enzyme during the six-hour incubation was 53% - 58% and 55.4% - 69.3%, respectively.