Abstract

Abstract Equal half-root systems of cowpea seedlings were achieved with a split-root technique. By use of a high humidity chamber and growth pouches, it was determined that the survival rate of seedlings with bisected root systems was greater than 95%, and the development of the half root systems was uniform and adequate. There were no marked differences in growth and development of plants when treatments were applied to different halves of the root system. However, there was evidence of Zn translocation from noninoculated half-root systems which received Zn, to inoculated half-roots not receiving Zn, which resulted in increased nodulation and N2 fixation. This result supports reports of a direct relationship of Zn nutrition to nodulation and N2 fixation.

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