Abstract
An analytical protocol is described which allows for the determination of polycyclic aromatic hydrocarbons (PAH) and phospholipid fatty acids on the same sample. This procedure allowed for the simultaneous evaluation of PAH concentration and its biological effects on the microbial community. The total extract was separated into PAH, neutral lipid, glycolipid and phospholipid fractions on a silicic acid column by sequential elution using hexane, chloroform, acetone and methanol. This method was shown to be simple, effective and reproducible, and can be applied to very small samples (20–100 mg of sediment). PAH concentration was determined directly from the hexane fraction by GC/MS analysis without further preparative steps. Microbial community structure was simultaneously determined using phospholipid ester-linked fatty acids (PLFA). This method was evaluated based on analysis of Standard Reference Material (NIST SRM 1941a), and applied to environmental samples from Eagle Harbor, Puget Sound, Washington.
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