The use of 86rubidium for the determination of changes in membrane permeability in guinea pig atrial tissue.

Abstract

Investigations were performed in order to establish whether86Rb may be used as a tool for the determination of changes in K-efflux, in guinea pig atrial tissue (isolated atria or left auricles). The isolated organs were incubated in K-free, Rb-containing Tyrode solution that contained 2.7 mM RbCl/l instead of KCl. An experimental, method was developed for the continuous recording of the mechanogram and of86Rb loss from isolated organs that had been loaded previously with86Rb.