Abstract
AbstractReversed‐phase HPLC separation of ionic dyes (methylene blue, neutral red, methyl orange, and acid orange) with 1‐alkyl‐3‐methylimidazolium ionic liquids as the eluent additives was studied. The addition of ionic liquid to the eluent affects the resolution, peak shape and reproducibility of the retention of ionic solutes. The use of ionic liquids as the additives facilitates the separation of ionic dyes with a reversed‐phase HPLC. Baseline‐resolved separation of these dyes was achieved in 12 minutes when the mobile phase of acetonitrile‐water (28:72,v/v) contained 0.3‐2.0% (v/v) [emim][BF4]. The detection limits for the four dyes ranged from 4.45 to 10.0 μg/L for an injection volume of 20 μL. Apparently, ionic liquid is an effective mobile phase additive for the separation of ionic dyes in reversed‐phase HPLC separation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
