THE UPTAKE OF RADIOACTIVE PHOSPHORUS INTO THE ACID-SOLUBLE COMPOUNDS OF GUINEA-PIG LIVER DURING ANAPHYLACTIC REACTION

Abstract

Previously, it was reported that in in vivo anaphylaxis there were observed a fall in adenosine triphosphate (ATP) level and a rise in adenosine monophosphate (AMP) level of guinea-pig liver, but no changes in the values of ATP and other adenine nucleotides in the lung of in vivo shock. However, the fall of ATP in liver was not observable in in vitro anaphylactic reaction (1). The fall of ATP level in tissues has been also reported in the animals treated with certain drugs (2-5) or with X-ray irradiation (6, 7). In the latter case, aside from a fall of ATP level, a significant decrease is shown in the P32 incorporation into nucleotide fraction of spleen and thymus in the mice transplanted with mammary carcinoma. The present paper describes the result of an investigation on the adenine nucleotide contents and on the P32 incorporation into the nucleotides of guinea-pig liver in in vivo anaphylaxis, as determined by the ion exchange chromatographic method. The experiments were also conducted on the animals treated with histamine, since histamine is known as a mediator in anaphylaxis in guinea pig, producing anoxia by constricting bronchial muscle, which is a phenomenon practically undistinguishable from anaphylactic shock.