Abstract
The epidermis of neonatal rat skin is separated from the dermis with trypsin, and preparations of the four cell strata are obtained by elution with EDTA or trypsin. The four preparations contain a predominance of each of the four cell types and are adequate for the examination of protein synthesis at the different levels of epidermis. The preparations were tested for protein synthesizing activity in two ways: 1) time course of incorporation of 14 C-histidine and 14 C-leucine by whole cells, and 2) their distribution in the buffer (BSS) soluble, NaOH soluble and NaOH insoluble protein fractions obtained from homogenized cell suspensions. The specific activity ranking of the preparations was spinous cells > basal cells > granular cells. The cornified cells were inactive. Early granular cells incorporated 14 C-histidine preferentially into their NaOH insoluble fraction.
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