The upregulation of miR-3945 in CD14+ monocytes is the risk factor for cardiovascular disease.

Abstract

Patients with type 1 diabetes (T1DM) show an increased risk of cardiovascular disease. Bioinformatics analysis revealed that it is characterized by changes in the function of CD14+ mononuclear macrophages. The current study was to explore the potential relationship between the miR-3845/TRIM35/PKM2 and abnormal polarization of mononuclear macrophages. Using bioinformatics to analyze the gene expression of mononuclear macrophages. The polarization of macrophages was analyzed using flow cytometry, and the expression changes of TRIM35/PKM2 were analyzed using Western blot, luciferase activity assay, and co-immunoprecipitation. Database analysis showed that T1DM patients showed an abnormal increase of miR-3945 in CD14+ monocyte macrophages. miR-3945 targets TRIM35 to release PKM2 to cytometry, and PKM2 causes M1-like polarization of mononuclear macrophages. Database analysis showed that miR-3945 was abnormally upregulated in CD14+ monocytes in T1DM patients. miR-3945 upregulates the expression of PKM2 in the cytoplasm by targeting TRIM35, which leads to M1 polarization of macrophages.