Abstract
KlUpc2p, atranscription factor belonging to the fungal binuclear cluster family, is an important regulator of ergosterol biosynthesis and azole drug resistance inKluyveromyces lactis.In this work, we show that the absence ofKlUpc2p generates Rag-phenotype and modulates theK. lactissusceptibility to oxidants and calcofuor white. TheKlUPC2deletion leads to increased expression of KlMGA2gene,encoding an important regulator of hypoxic and lipid biosynthetic genes inK. lactis and also KlHOG1 gene. The absence ofKlUpc2p does not lead to statistically significant changes in glycerol, corroborating the expression ofKlGPD1gene, encoding NAD+-dependent glycerol-3-phosphate dehydrogenase, that is similar in both the deletion mutant and the parental wild-type strain. Increased sensitivity ofKlupc2mutant cells to brefeldin Aaccompanied with significant increase inKlARF2gene expression point to the involvement ofKlUpc2p in intracellular signaling. Our observations highlight the connections between ergosterol and fatty acid metabolism to modulate membrane properties and point to the possible involvement of KlUpc2p in K. lactis oxidative stress response.
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