Abstract
Mitochondria were isolated from normal rat and mouse liver and kidney, hepatocytes, ascites tumor cells, and livers of rats treated in various ways known to affect the size of liver mitochondria. These mitochondria were then distributed by particle size through a linear sucrose-density gradient in a zonal centrifuge, and the distribution of ornithine aminotransferase activity was compared to that of malate dehydrogenase activity. While the activities of these enzymes are congruent in mitochondria prepared from several tissues other than liver, the midpoints of their distributions are consistently different in mitochondria prepared from whole liver and from hepatocytes. The changes in the separation of midpoints in animals treated in various ways (subtotal hepatectomy, corticosteroid administration, and diet modification) are consistent with the histologic effects of these treatments on liver mitochondria, and suggest that ornithine aminotransferase activity may prove a useful marker for a unique population of mitochondria perhaps localized in cells near the central vein.
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