The unfolded-protein-response sensor IRE-1α regulates the function of CD8α+ dendritic cells

Abstract

Event Abstract Back to Event The unfolded-protein-response sensor IRE-1α regulates the function of CD8α+ dendritic cells Fabiola Osorio1*, Simon Tavernier2, Sophie Janssens2 and Bart N. Lambrecht2 1 Universidad de Chile, Institute of Biomedical Sciences, Faculty of Medicine, Chile 2 VIB-Ghent University, Belgium The role of the unfolded protein response (UPR) and endoplasmic reticulum (ER) stress has been linked to the initiation of several inflammatory diseases whereas their contribution in homeostasis of the immune system remains incompletely understood. Here, we show that dendritic cells (DCs) constitutively activate the UPR sensor Inositol-requiring enzyme 1-α (IRE-1α) and its target transcription factor X-box-binding protein 1 (XBP-1) in the absence of a canonical ER stress response. Selective ablation of XBP-1 in CD11c+ cells led to defects in phenotype, ER homeostasis and antigen presentation by conventional CD8α+ DCs, yet closely related CD11b+ DCs were unaffected. Whereas the dysregulated ER resulted from loss of XBP-1 transcriptional activity, the phenotypic and functional defects resulted from regulated IRE-1α dependent degradation (RIDD) of mRNAs for CD18 integrins and MHC class I machinery. Thus, a precisely regulated feedback circuit involving IRE-1α and XBP-1 controls the homeostasis of CD8α+ cDCs. Acknowledgements Funding: VIB-Ugent, ERC,FEBS and Marie Curie fellowships. Currently financed by grant CONICYT, PAI, No 82130031 /016-F. Keywords: Dendritic Cells, UPR signaling pathways, XBP-1, IRE1α, Antigen Presentation Conference: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología, Medellin, Colombia, 13 Oct - 16 Oct, 2015. Presentation Type: Oral Presentation Topic: Innate Immunity Citation: Osorio F, Tavernier S, Janssens S and Lambrecht BN (2015). The unfolded-protein-response sensor IRE-1α regulates the function of CD8α+ dendritic cells. Front. Immunol. Conference Abstract: IMMUNOCOLOMBIA2015 - 11th Congress of the Latin American Association of Immunology - 10o. Congreso de la Asociación Colombiana de Alergia, Asma e Inmunología. doi: 10.3389/conf.fimmu.2015.05.00281 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 15 May 2015; Published Online: 15 Sep 2015. * Correspondence: PhD. Fabiola Osorio, Universidad de Chile, Institute of Biomedical Sciences, Faculty of Medicine, Santiago, Chile, fabiolaosorio@med.uchile.cl Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Fabiola Osorio Simon Tavernier Sophie Janssens Bart N Lambrecht Google Fabiola Osorio Simon Tavernier Sophie Janssens Bart N Lambrecht Google Scholar Fabiola Osorio Simon Tavernier Sophie Janssens Bart N Lambrecht PubMed Fabiola Osorio Simon Tavernier Sophie Janssens Bart N Lambrecht Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.