The underlying mechanism of A-type procyanidins from peanut skin on DSS-induced ulcerative colitis mice by regulating gut microbiota and metabolism.

Abstract

Ulcerative colitis (UC) is a kind of inflammatory bowel disease. Procyanidins have been found to prevent UC. However, most research has been focused on the alleviation effect of B-type procyanidins on UC and ignored those of A-type procyanidins. Hence, this study aims to investigate the anti-UC effect and the potential mechanism of A-type procyanidins by combining gut microbiome and metabolic profile. UC was induced by dextran sulfate sodium (DSS) in Balb/c mice, and then the mice were administrated with peanut skin procyanidins (PSP; rich in A-type procyanidins) for 9 days. Administration of PSP can ameliorate DSS-induced UC by mediating the intestinal barrier, the expression of inflammatory cytokines (TNF-α, IL-β, IL-6, and IL-10) and oxidative stress (MDA, T-SOD, NO, and iNOS) in mice. We observed that PSP affects the gut microbiota and colon metabolomic patterns of mice. The 16S rDNA sequencing showed increase in abundance of Lachnospiraceae_NK4A136_group, Oscillibacter and Roseburia and decrease of Bacteroides, Helicobacter, Parabacteroides, Escherichia-Shigella, and Enterobacter after PSP treatment. The colon tissue metabolome was significantly altered, as reflected by regulating taste transduction, mTOR signaling pathway, PI3K-Akt signaling pathway, and FoxO signaling pathway to improve the protection against UC. PRACTICAL APPLICATIONS: We investigated the anti-ulcerative colitis (UC) effect and its potential mechanism of peanut skin procyanidins (PSP). This suggests that PSP with abundant A-type procyanidins may be an effective candidate for dietary supplementation to alleviate the symptoms of UC by regulating gut microbiota and metabolism.