Abstract
An ultraviolet spectrophotometric method of assay for phenobarbital in official phenobarbital preparations is described. Procedures are presented utilizing the optical density of phenobarbital solutions in a borate buffer at pH 9.5 to obtain assay results. The results of 21 assays are reported with satisfactory agreement with known or labeled values. The assay method is shown to be applicable to phenobarbital tablets containing stearates. A change in the absorption characteristics of phenobarbital allowed to stand in 2 per cent sodium hydroxide is reported.
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