Abstract
It is now clearly established that bacterial transformation is mediated by deoxyribonucleic acid (DNA). The effect of ionizing radiation on pneumococcus transforming DNA has been reported (1-3). However, although the effect of ultraviolet light on the transforming DNA of Hemophilus influenzae has been studied extensively (4, 5), there are few studies of the pneumococcal transforming system (6). This report concerns the effect of ultraviolet irradiation (UV) on the streptomycin-transforming activity of pneumococcus DNA and of fractions obtained by chromatography on the anion exchanger ECTEOLA-cellulose (7). Unfractionated DNA was heterogeneous in its UV sensitivity. Fractions differed from the starting material in the degree of heterogeneity, and one of those studied proved to be homogeneous.
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