The ultrastructure of the sensory hairs of the paratympanic organ receptor cells in chicken.

Abstract

The hair bundle of the receptor cells in the paratympanic organ of the chicken was studied by TEM, after fixation in aldehydes/osmium tetroxide or in aldehydes/osmium tetroxide/tannic acid. The bundles are formed by a kinocilium and by 40-70 stereocilia. The stereocilia are linked to each other by an extensive network of filaments. Three types of these connectors are present: basal, shaft and apical; the latter consist of side-to-side and tip-to-side connectors. We observed that the shaft connectors are well-highlighted only when tannic acid was used, while the other connectors are to be found in the conventionally fixed specimens also. The tip-to-side connector consists of a filament which joins the tip of a stereocilium with the side of an adjacent taller stereocilium; we suggest that the distortion of this filament would give rise to the mechanosensory transduction. The other connectors probably serve to maintain the regular spatial arrangement of the hair bundle and the mechanical coupling of the stereocilia. Our study shows that the general conformation of the hair bundle and the stereociliary links of the hair cells in the paratympanic organ of chicken are similar to those previously described in the hair cells of the acoustico lateralis system.