Abstract
The ultrastructure of two lymphoblastoid cell lines derived from Marek's disease lymphomata has been studied. The cells varied from 5 to 12 mum in diameter and had large round or oval nuclei. A nucleolus was occasionally present and about 3% of cells showed projections of the nuclear envelope. The cytoplasm contained many ribosomes and several mitochondria but endoplasmic reticulum was sparse. A small number of cells contained annulate lamellae and crystalline structures were occasionally seen. Cells with immature intranuclear herpesvirus particles were rarely present. The cells had many ultrastructural features in common with Burkitt's lymphoma-derived cell lines.
Highlights
Summary.-The ultrastructure of two lymphoblastoid cell lines derived from Marek's disease lymphomata has been studied
Ovarian lymphomata were collected from Houghton Poultry Research Station (HPRS) Rhode Island Red chickens experimentally infected at one day of age with a virulent strain of Marek's disease virus (HPRS-16)
In smears and cytocentrifuge preparations stained with May-GriinwaldGiemsa stains (Fig. 1 ) the cells had large nuclei an(d a rim of intenselv staining basophilic cytoplasm containing vacuoles
Summary
Ovarian lymphomata were collected from Houghton Poultry Research Station (HPRS) Rhode Island Red chickens experimentally infected at one day of age with a virulent strain of Marek's disease virus (HPRS-16). In smears and cytocentrifuge preparations stained with May-GriinwaldGiemsa stains (Fig. 1 ) the cells had large nuclei an(d a rim of intenselv staining basophilic cytoplasm containing vacuoles. When examined in the electron microscope, the cells were usually round (Fig. 2, 3) or sometimes oval in shape, a few were elongated (Fig. 3). Several mitochondria were present in the cytoplasm but the endoplasmic reticulum, which was usually rough surfaced, was sparse (Fig. 2, 3). Often irregularly shaped osmiophilic bodies, which were probably composed of lipid, were present in most of the cells Z.-Electron micrograph of a cell with a contains ribosomes, mitochondria, a small surfaced cisternae of endoplasmic reticulum large lipid (er). 3.-A round c-e-lI l containing few cytoplasmic organelles adjacent cell is elongated and contains many lipid bodies arranged round the periphery of the nucleus, and small.
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