Abstract
The insectivorous, nocturnal prosimian, Galago senegalensis (bushbaby), relies on its arboreal running and leaping ability for survival, and extreme hand-eye coordination for prey capture. In mammals, these functions require a well developed motor system mediated by a highly evolved cerebellum. The mammalian cerebellar cortex is characterized by a three layer configuration comprised of the molecular, Purkinge, and granular layers. In this study, we examined the ultrastructure of the cerebellar cortex of the bushbaby.A six year old male bushbaby was anesthetized with 2 cc sodium pentobarbitol injected intraperitoneally. The animal was perfused through the superior vena cava by flushing the system with saline and 2% heparin, followed by fixation with 2% glutaraldehyde for 30 min. After primary fixation, the animal was decapitated and the head placed in 2% glutaraldehyde overnight. The brain was removed and desired areas were excised from the brain, post-fixed with osmium tetroxide, dehydrated in acetone, and embedded in Epon Araldite.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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