The UK National External Quality Assessment Scheme in Blood Group Serology. Compatibility testing 1979-1980: trends and performance in relation to practice in antiglobulin testing

Abstract

Surveys of antiglobulin test procedures and reagents were undertaken in 1979-1980 as part of a national external quality assessment scheme in compatibility testing. An extraordinary lack of standardization was revealed. In addition, practices underwent considerable changes over this period. The use of tube and of low ionic strength solution (LISS) techniques increased whilst the use of tile and of albumin-antiglobulin techniques declined. Performance in compatibility test exercises was significantly better with tube techniques than with tile techniques in 9/22 incompatibilities. Performance with LISS techniques was occasionally significantly better than with normal ionic strength techniques. Performance with albumin-antiglobulin techniques was occasionally significantly worse than in the absence of albumin. To varying extents significant relationships were found between performance and cell concentration, serum/cell concentration ratio, antiglobulin reagent and method of reading the results.