Abstract
P1197 Aims: Shortage of cadaver donors is a problem also for islet transplantation. Recent improvements in isolated islet transplantation have led to a renewed interest in the use of non-heart-beating (NHB) donors. NHB donors could provide a significant source of pancreata for islet transplantation, but have not previously been assessed as a source of islets for transplantation since the additional factor of warm ischemic injury adversely affects the success of islet isolation. In the current study, we tested whether the two-layer method (TL) could improve islet yield and function from damaged pancreata after warm ischemia (WI). Methods: Male Lewis rats were divided into 8 groups. In groups 1 to 4, rats were subjected to 0, 30, 45 and 60 minutes of WI, respectively. WI insult was created by placing animals in 37°C warm bath for designated periods. Subsequently, islets were isolated immediately (subgroup a) or after 3-hour preservation with TL (subgroup b). Before this isolation, pancreatic tissue adenosine triphosphate (ATP) concentration was determined by HPLC. Isolated islets were assessed in terms of islet yield and function (stimulation index). Results: Table summarizes the tissue ATP levels and islet yields in all groups.FigureTL preservation increased the ATP levels in groups 2, 3 and 4. This was more pronounced in groups 2 and 3. Islet yield decreased after WI (groups 2a and 3a). However, TL doubled the yield in group 2 while mealy a small increase was observed in group 3. Increase in ATP levels did not parallel one in islet yield. Stimulation indices in groups 1-a, 2-a and 2-b were 1.66±1.06, 1.14±0.55 and 1.52±0.90, respectively. Conclusions: Islet yield and function results from this experimental study demonstrated that pancreata damaged by warm ischemia for 30 or less minutes were restored with 3-hour TL preservation before isolation. TL may allow the use of NHB donors as an alternative source of islets for transplantation.
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