Abstract
Abstract Mice were treated twice daily for 4 days with injections of sodium phenobarbital, 75 mg per kg. There was a 25% increase in liver weight, a 50% increase in the amount of microsomal protein, and a 20% increase in the amount of soluble protein. When tritiated l-leucine was injected 24 hours before the start of treatment, the half-time for loss in vivo of radioactivity from microsomal protein was 3.5 days in control and 7 days in phenobarbital-treated mice. There was little change in specific activity of microsomal protein. The 90-min incorporation of l-leucine-3H into the microsomal protein of mice that had been pretreated for 2 or 3 days with sodium phenobarbital was increased about 20%. The accumulation of liver protein in animals treated with barbiturates seems to result from both a decreased rate of breakdown and an increased rate of synthesis.
Highlights
Mice were treated twice daily for 4 days with injections of sodium phenobarbital, 75 mg per kg
Changes in Liver Weight and Protein Content-When mice received injections twice daily of 75 mg per kg of sodium phenobarbital for 4 days, there was an increase of approximately 25% in liver weight and 50% in total microsomal protein (Fig. 1)
The amount of total soluble protein per liver increased about 22y0 as a result of 4 days of treatment with phenobarbital (Table I). This change is in accord with the observations that in rats treated with phenobarbital the increase in total liver protein exceeds the change in microsomal protein (1) and that there are increased levels of some soluble liver enzymes (14)
Summary
Mice were treated twice daily for 4 days with injections of sodium phenobarbital, 75 mg per kg. When tritiated L-leucine was injected 24 protein. We have measuredthe turnover of microsomapl rotein in the livers of mice treated with phenobarbital. The rate of protein breakdown was decreasedby treatment with phenobarbital. Hours before the start of treatment, the half-time for loss in vivo of radioactivity from microsomal protein was 3.5 days in control and 7 days in phenobarbital-treated mice. The QO-minincorporation of L-leucine-3Hinto the microsomal protein of mice that had been pretreated for 2 or 3 days with sodium phenobarbital was increased about 20%. The accumulation of liver protein in animals treated with barbiturates seems to result from both a decreased rate of breakdown and an increased rate of synthesis
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