The tumor necrosis factor-α gene polymorphism (-308g/a) in type 2 diabetes mellitus patients with tuberculosis infection

Abstract

The tumor necrosis factor (TNF)-a gene polymorphism (-308G/A) has been shown influence several diseases. This study aims to analyze the distribution of the TNF-α gene polymorphism (-308G/A) in type 2 diabetes mellitus (T2DM) patients with tuberculosis infection. Forty of T2DM patients with tuberculosis infection were recruited at Balai Pengobatan Lung Disease, Medan. Data collection of characteristics subjects were done through interviews using questionnaires. The blood glucose were measured with a spectrophotometer at a wavelength of 500 nm. Nco1 restriction enzyme was used to digested of gene polymorphism (-308G/A). Genotype frequency of the TNF-α gene was analyzed by direct counting. In this study shown that the TNF-α gene polymorphism (-308G/A) had 38 (95%) of G/G genotype and 2 (5%) of G/A genotype. No A/A genotype shown in this population. This preliminary result indicated that G/G genotype was common genotype in the TNF-α gene polymorphism (-308G/A) in T2DM patients with tuberculosis infection.