Abstract
Southern analysis of Triticum DNA has revealed that nuclear tRNA Tyr genes are dispersed at a minimum of 16 loci in the genome. We have isolated six independent tRNA Tyr genes from a Triticum aestivum library in addition to three known members of the Triticum tRNA Tyr family. Four of the sequenced tRNA Tyr genes code for Triticum tRNA 1 Tyr and two code for tRNA 2 Tyr. Three genes encode tRNA Tyr which carry one or two nucleotide substitutions as compared to the conventional genes. The nine Triticum tRNA Tyr genes possess highly conserved intron sequences ranging in size from 12 to 14 nucleotides. A common secondary intron structure with the 5′ and 3′ splice site loops separated by five base pairs can be formed by all pre-tRNAs Tyr which are efficiently spliced in the homologous wheat germ extract.
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