Abstract
Carbamyl phosphate synthetase-I (CPS-I) 2 2 Abbreviations used: CPS-I, carbamyl phosphate synthetase-I; pCPS-I, precursor of carbamyl phosphate synthetase-I; EDTA, Disodium ethylene diamine tetraacetate; CCCP, carbamyl cyanide m-chlorophenyl hydrazone; SDS, sodium dodecyl sulfate; PMSF, phenylmethyl sulfonyl fluoride; TLCK, N α-p-tosyl-L-lysine chloromethyl ketone; TPCK, L-1-p-Tosylamino-2-phenylethylchloromethyl ketone , purified from mouse hepatic mitochondria consists of electrophoretically homogeneous polypeptide species of 160 kilodaltons molecular weight (Kd). Monospecific antibody to CPS-I immunoprecipitated a putative precursor of 165Kd protein from in vitro translation products programmed with mouse liver free polysomes or poly(A) RNA. Isolated mitochondrial particles can take up and process pCPS-I into mature CPS-I of 160Kd in an in vitro transport system. The in vitro transport of CPS-I is energy dependent and requires intact mitochondria. The processing of pCPS-I appears to involve a single endoproteolytic cleavage.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
