The translation machinery and 70 kd heat shock protein cooperate in protein synthesis

Abstract

The function of the yeast SSB 70 kd heatshock proteins (hsp70s) was investigated by a variety of approaches. The SSB hsp70s ( Ssb1 2p ) are associated with translating ribosomes. This association is disrupted by puromycin, suggesting that Ssb1 2p may bind directly to the nascent polypeptide. Mutant ssb1 ssb2 strains grow slowly, contain a low number of translating ribosomes, and are hypersensitive to several inhibitors of protein synthesis. The slow growth phenotype of ssb1 ssb2 mutants is suppressed by increased copy number of a gene encoding a novel translation elongation factor 1α (EF-1α)-like protein. We suggest that cytosolic hsp70 aids in the passage of the nascent polypeptide chain through the ribosome in a manner analogous to the role played by organellelocalized hsp70 in the transport of proteins across membranes.