Abstract
Deoxyribonucleic acid of λdg, isolated by phenol extraction, will transform galactose-negative strains of Escherichia coli K12. That the transforming agent is indeed DNA is shown by (1) its sensitivity to pancreatic DNase, (2) its resistance to anti-λ antibody, (3) its resistance to heat up to the characteristic melting temperature of λdg DNA, and (4) its buoyant density of 1·71 g cm−3. Transformation is found to occur only if the bacteria exposed to λdg DNA are simultaneously infected with ordinary λ. The transforming agent appears to be the entire λdg chromosome since the phage genes c and mi are also present in the galactose-positive transformants.
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