Abstract
‘Polima’ cytoplasmic male sterility (cms) was transferred in one step from spring to winter lines of oilseed rape ( Brassica. napus L.) by protoplast fusion. The transfer was confirmed by restriction fragment length polymorphism (RFLP) analysis of cytoplasmic DNA. Amphidiploid individuals with normal female fertility were recovered. The regenerated plants retained the winter habit. All traits studied were stable through subsequent sexual generations. The introduction of cms to winter lines by a conventional backcross normally takes 3 years; a similar alloplasmic substitution was achieved in approximately 9 months by protoplast fusion.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
