The transcriptomes of novel marmoset monkey embryonic stem cell lines reflect distinct genomic features.

Katharina Debowski,Ralf Dressel,Charis Drummer,Maren Cors,Erika Sasaki,Jana Lentes,Rüdiger Behr,Sigrid Fuchs,Thomas Lingner,Gabriela Salinas-Riester

doi:10.1038/srep29122

Abstract

Embryonic stem cells (ESCs) are useful for the study of embryonic development. However, since research on naturally conceived human embryos is limited, non-human primate (NHP) embryos and NHP ESCs represent an excellent alternative to the corresponding human entities. Though, ESC lines derived from naturally conceived NHP embryos are still very rare. Here, we report the generation and characterization of four novel ESC lines derived from natural preimplantation embryos of the common marmoset monkey (Callithrix jacchus). For the first time we document derivation of NHP ESCs derived from morula stages. We show that quantitative chromosome-wise transcriptome analyses precisely reflect trisomies present in both morula-derived ESC lines. We also demonstrate that the female ESC lines exhibit different states of X-inactivation which is impressively reflected by the abundance of the lncRNA X inactive-specific transcript (XIST). The novel marmoset ESC lines will promote basic primate embryo and ESC studies as well as preclinical testing of ESC-based regenerative approaches in NHP.

Highlights

Embryonic stem cells (ESCs) are useful for the study of embryonic development
Due to the close phylogenetic relationship of non-human primates (NHP) to humans, NHP ES cells represent an excellent alternative to human ES cells with regard to basic and preclinical ES cell research reviewed in refs 13,14 and allow experiments not possible with human embryos[15]
We report the derivation of four novel embryonic stem cell lines from natural preimplantation embryos of the common marmoset monkey (Callithrix jacchus)

Summary

Introduction

Embryonic stem cells (ESCs) are useful for the study of embryonic development. since research on naturally conceived human embryos is limited, non-human primate (NHP) embryos and NHP ESCs represent an excellent alternative to the corresponding human entities. We report the generation and characterization of four novel ESC lines derived from natural preimplantation embryos of the common marmoset monkey (Callithrix jacchus). The novel marmoset ESC lines will promote basic primate embryo and ESC studies as well as preclinical testing of ESC-based regenerative approaches in NHP. If cultured under appropriate conditions, ES cells can proliferate indefinitely while maintaining a pluripotent state[1] They can give rise to all somatic cell types deriving from the embryonic germ layers, i.e. ectoderm, mesoderm and endoderm, as well as germ cells. Due to the close phylogenetic relationship of non-human primates (NHP) to humans, NHP ES cells represent an excellent alternative to human ES cells with regard to basic and preclinical ES cell research reviewed in refs 13,14 and allow experiments not possible with human embryos[15]. The marmoset monkey is small, easy www.nature.com/scientificreports/

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