The Transcriptional Regulatory Network of Corynebacterium glutamicum

Abstract

AbstractOver the past few years, the transcriptional regulation of gene expression was intensively studied in the Gram-positive model organism Corynebacterium glutamicum to shed light on its gene regulatory repertoire and the architecture of its transcriptional regulatory network. The combination of several computational methods revealed a set of at least 159 regulatory proteins, which form the minimal transcriptional regulatory repertoire of the type strain C. glutamicum ATCC 13032. Most of these regulatory proteins have a direct role as a DNA-binding transcription regulator or sigma factor, while others have less well-defined functions in transcriptional regulation. Considerable information on 88 transcription regulators has been accumulated and stored in the online reference database CoryneRegNet, leading to a data set of more than 1,000 interactions between regulatory proteins and their target genes. Based on this comprehensive collection of gene-regulatory data, we have achieved great improvements in understanding the regulatory and coregulatory interactions of the various transcription regulators, their connection by hierarchical cross-regulation, and the topology of the transcriptional regulatory network. The genome-wide reconstruction of the transcriptional regulatory network with the graph visualization feature of CoryneRegNet reveals a highly connected architecture that displays a modular and hierarchical structure without feedback regulation at the transcriptional level.KeywordsTranscription RegulatorCorynebacterium GlutamicumTranscriptional Regulatory NetworkArsenate ReductaseBacterial Genome SequenceThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.