Abstract
As examined by the luciferase assay, a negative regulatory region including the NFκB motif was found in the 5′-flanking region of the 12-lipoxygenase gene in human erythroleukemia cells. The negative control was abolished by a site-specific mutation of the NFκB motif. Probes including the NFκB region gave positive bands upon a gel-shift assay. The bands were supershifted by antibodies for NFκB p50, NFκB p65 and c-Rel, and were lost by a NFκB competitor DNA. Furthermore, the NFκB sequence was protected in DNase I footprinting. Thus, two kinds of heterodimer (p50 and p65; p50 and c-Rel) seemed to control the over-expression of the human 12-lipoxygenase gene.
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