Abstract
Beetles, Tribolium castaneum, were sprayed with mixtures of pyrethrins and piperonyl butoxide in non-volatile oil. The results were fitted to the equation y = a + b 1 log z 1 + b 2z 2 (c + z 2) , where y is probit mortality, z 1 = 10 × pyrethrin concentration, and z 2 = piperonyl butoxide concentrations; a, b 1, b 2, and c are parameters. Having estimated the parameters it was possible to deduce the relations between probit mortality and log-concentration of pyrethrins for different proportions of piperonyl butoxide to pyrethrins, and to construct isoboles, that is curves showing the relations between pyrethrin concentrations and piperonyl butoxide concentrations giving fixed levels of mortality. For indefinitely large doses of piperonyl butoxide the mixtures were estimated to be 3·04 times as toxic as pyrethrins alone.
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