Abstract
An ‘alternating solution’ culture method was used to study the effects of chloride ions and humic acid (HA) on the uptake of cadmium by barley plants. The plants were transferred periodically between a nutrient solution and a test solution containing one of four levels of HA (0, 190, 569 or 1710 μg cm−3) and one of five levels of Cd (0, 0.5, 1.0, 2.5 or 5.0 μg cm−3) in either a 0.006M NaNO3 or 0.006M NaCl medium. Harvest and analysis of shoots and roots was after nineteen days. The distribution of Cd in the test solutions between Cd2+, CdCl+ and HA-Cd was determined in a separate experiment by dialysis equilibrium.
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