Abstract
The total N-acetyl neuraminic acid (NANA) content of lymphocytes, erythrocytes and plasma obtained from normal and lymphatic leukaemic patients was measured by the Warren thiobarbiturate technique. Lymphocytes from patients with chronic lymphatic leukaemia and lymphoblasts from patients with acute leukaemia had decreased levels.
Highlights
N-ACETYL neuraminic acid (NANA) contributes to the structure of various oligo and polysaccharides, glycolipids and glycoproteins, which are distributed widely throughout the cell
The total NANA content of lymphocytes, as isolated by PVP sedimentation and glass wool filtration and the triosil/ ficoll technique was estimated in 9 subjects, 7 of whom were controls and 2 of whom had chronic lymphatic leukaemia
It was concluded that the NANA content of lymphocytes did not depend on the method of isolation and as the triosil/ ficoll method was quicker, giving a yield of 70% and a purity of 98% as opposed to the PVP method which gave a yield of only 25% and a purity of 950o, the
Summary
N-ACETYL neuraminic acid (NANA) contributes to the structure of various oligo and polysaccharides, glycolipids and glycoproteins, which are distributed widely throughout the cell. Whilst little is known about the intracellular distribution, it is established that the amount bonded to the plasma membrane influences surface charge density (Eylar et al, 1962) and the adhesiveness of various cell types (Pethica 1961). Rueff, Fuhrmann and Ruhenstroth-Bauer (1963) found that lymphocytes from patients with chronic lymphatic leukaemia (CLL) had a higher surface charge density than normal lymphocytes. Lichtman and Weed showed that the NANA content of the plasma membranes in normal and CLL lymphocytes were similar. In this paper we report observations on the content of total cellular NANA in lymphocytes from patients with CLL, as compared with lymphocytes from normal subjects and have extended these observations to erythrocytes and plasma; studied were the lymphoblasts from 3 cases of acute lymphatic leukaemia (ALL)
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