Abstract
Background: Among its many uses, Acmella oleracea (L.) R. K. Jansen as an anthelmintic agent for general intestinal helminthiasis is remarkable because of its indigenous usage in the Mizo traditional medicine. However, the rationale has not been established. Objectives: The objective of this study is to perform chemical analysis of the plant extract using gas chromatography-mass spectrometry (GC-MS) and to test the anthelmintic activity on intestinal helminths. Materials and Methods: Methanol extract of the whole plant was prepared and volatile compounds were analyzed using GC-MS. Anthelmintic activity was studied by survival test on the cestode Raillietina echinobothrida and the nematode Ascaridia galli. Anthelmintic effects were examined using scanning electron microscopy. Results: Thirteen compounds were detected, with methyl n-hexadecanoate (palmitic acid) as the major constituents. N-Alkylamides such as N-isobutyl-(2E,4Z,8Z,10E)-dodecatetraenamide and N-(2-phenylethyl) non-2(E)-en-6, 8-diynamide were present. The plant extract was significantly effective (P
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