The tomato floral homeotic protein FBP1-like gene, SlGLO1, plays key roles in petal and stamen development.

Xuhu Guo,Xiaohui Yu,Zhiguo Zhu,Wencheng Yin,Zongli Hu,Guoping Chen,Jianling Zhang

doi:10.1038/srep20454

Abstract

MADS-box transcription factors play important role in plant growth and development, especially floral organ identities. In our study, a MADS-box gene SlGLO1- tomato floral homeotic protein FBP1-like gene was isolated. Its tissue-specific expression profile analysis showed that SlGLO1 was highly expressed in petals and stamens. RNAi (RNA interference) repression of SlGLO1 resulted in floral organ abnormal phenotypes, including green petals with shorter size, and aberrant carpelloid stamens. SlGLO1-silenced lines are male sterile. Total chlorophyll content was increased and chlorophyll biosynthetic genes were significantly up-regulated in SlGLO1-silenced petals and stamens. Furthermore, B-class genes expression analysis indicated that the repressed function of SlGLO1 led to the enhanced expression of TAP3 and the down-regulation of TPI in the petals and stamens, while the expression of TM6 was reduced in petals and increased in stamens and carpels of SlGLO1-RNAi plants. Additionally, pollen grains of transgenic lines were aberrant and failed to germinate and tomato pollen-specific genes were down-regulated by more than 90% in SlGLO1-silenced lines. These results suggest that SlGLO1 plays important role in regulating plant floral organ and pollen development in tomato.

Highlights

ABC model of flower development was broadly accepted based on extensive genetic and molecular studies in several model plants[1]
In order to explore the potential function of MADS-box genes playing in reproductive organ development in tomato, we isolated a tomato floral homeotic protein FBP1-like gene from wild-type tomato flowers based on a cDNA clone
Nucleotide sequence analysis showed that SlGLO1 was consistent with the cDNA clone, and contained an open reading frame (ORF) of 633 nucleotides and encoded 210 amino acid residues with an estimated molecular mass of 24.74 kDa

Summary

Introduction

ABC model of flower development was broadly accepted based on extensive genetic and molecular studies in several model plants[1]. B-class MADS box genes specify petal and stamen identities in several core eudicot species, such as, Solanum lycopersicum GLOBOSA (SlGLO7) In model plant Antirrhinum, thresholds of 11–15% of wild-type levels of expression of DEF or GLO are associated with development of recognizable petal tissue[28], supporting the importance of quantitative gene expression levels for floral patterning and organ development. Ailsa Craig, as the research material which is normal height compared with Micro-Tom. To comprehensively examine the diversification of functions of B-class genes in the Solanaceae, We have isolated SlGLO1 gene (a tomato floral homeotic protein FBP1-like gene) from wild-type tomato This article enhanced our knowledge about the roles of SlGLO1 playing in diverse developmental processes

Methods

Results

Conclusion