The tiny mayfly in the room: implications of size-dependent invertebrate taxonomic identification for biomonitoring data properties

Abstract

The appropriate level of taxonomic identification, taxonomic sufficiency, for biomonitoring purposes continues to be controversial. Taxonomic sufficiency, however, fails to address the bias created by size-dependent taxonomic identification, which can result in coarse-resolution identification for immature specimens lacking distinguishing characteristics. Our study provides a direct test for this potential systematic bias in biomonitoring data by examining two morphological traits: body size and shape of key organisms (Ephemeroptera, Plecoptera, Trichoptera and Odonata) collected from standard aquatic biomonitoring samples. Direct measurement of body size and a geometric morphometric description of body shape provide consistent, quantitative variables to describe the composition of specimens identified at different levels of taxonomic resolution (genus or family). Corroborating our expectations, we observed evidence of systematic size bias in family-level identifications. Specimens that could only reliably be identified to the family level were significantly smaller than specimens identified to the genus level. Qualitative comparisons of shape variation between specimens demonstrated a high degree of variation in specimens identified only at the family level and support the conclusion that specimens identified at the family level possess multiple constituent taxa (genera or species). Thus, size-dependent taxonomy can have negative consequences for the accurate determination of biodiversity and may invalidate common biomonitoring metrics. Improvements to biomonitoring protocols through technological advances, including DNA-based taxonomy to augment specimen identification, should effectively remove the size-bias problem in the long term. In the short-term, recognizing instances of size bias, the degree to which it may impact bioassessment and exploring methods for remediation, including traits-based assessments, can enhance data quality and inferences derived from biomonitoring studies.