Abstract

The time dependence and efficiency of excitation of three-quantum (3Q) coherence is examined for the peaks that are commonly observed in 'H NMR 3Q spectra of proteins. The algebraic expressions for the various contributions to each 3Q coherence are tabulated. Plots of the excitation efficiency versus the length of the excitation period are shown. These results provide guidelines for optimizing the length of the 3Q excitation period to obtain either a wide range of 3Q signals in the spectrum or the excitation of specific 3Q signals with maximum efficiency.

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