The Tibetan herbal medicines Padma 28 and Padma Circosan inhibit the formation of advanced glycation endproducts (AGE) and advanced oxidation protein products (AOPP) in vitro.

Ewa Grzebyk,Agnieszka Piwowar

doi:10.1186/1472-6882-14-287

Abstract

BackgroundAdvanced glycation endproducts (AGE) and advanced oxidation protein products (AOPP) play a pivotal role in the development of diabetes associated diseases. The herbal medicines Padma 28 and Padma Circosan have shown effectiveness in symptoms of diabetes associated diseases and have antioxidant effects. It is not known whether inhibition of AGE and AOPP formation is a mechanism of their action.MethodBSA was subjected to glycation or oxidation with or without 70% ethanolic extracts of Padma 28, Padma Circosan or with an active control. AGE and AOPP concentrations were analyzed fluorimetrically or spectrophotometrically respectively and by ELISA.ResultsCompared to the positive control Padma 28, Padma Circosan and the active control significantly reduced AGE levels by 58.6%, 56.7%, and 8.14% (fluorimetry) and by 35.48, 34.19, and 19.68% (ELISA). AOPP were reduced by 57.28/66.78% (30’/60’ incubation), by 67.08/71.99%, and by 81.68/86.54% (spectrophotometry) or by 79.98/86.97%, 79.3/84.3% and 77.07/90.31% (ELISA). All results are significantly different (p < 0.001). No difference was found between the effects of the two preparations.ConclusionBoth formulas significantly inhibited the formation of AGE and AOPP to a similar extent as the active controls. This suggests a possible role for both Padma preparations in the treatment and prevention of diabetes associated diseases.

Highlights

Advanced glycation endproducts (AGE) and advanced oxidation protein products (AOPP) play a pivotal role in the development of diabetes associated diseases
No difference was found between the effects of the two preparations. Both formulas significantly inhibited the formation of AGE and AOPP to a similar extent as the active controls
Glycation process The ethanolic extracts of both Padma 28 and Padma Circosan as well as the active control inhibited the formation of AGE significantly as measured fluorimetrically as well as by ELISA

Summary

Introduction

Advanced glycation endproducts (AGE) and advanced oxidation protein products (AOPP) play a pivotal role in the development of diabetes associated diseases. The herbal medicines Padma 28 and Padma Circosan have shown effectiveness in symptoms of diabetes associated diseases and have antioxidant effects It is not known whether inhibition of AGE and AOPP formation is a mechanism of their action. Together with other factors such as inflammatory processes and dietary and genetic causes chronic hyperglycemia and oxidative stress are the two main factors causing metabolic disturbances in diabetes mellitus (DM) They lead to glycation and oxidation of macromolecules and tissues, thereby altering their structure and function. Intensive glucose lowering showed limited benefits regarding all cause mortality and deaths from cardiovascular causes in a meta-analysis [2] In view of these data and considering the importance of diabetes-associated diseases, an extended therapy and prevention approach is needed for diabetic patients. In view of the pivotal role diabetic complications play in the disease process and mortality of diabetes such preparations might be highly valuable in treatment and prevention of DM and its complications

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