The threat of foodborne disease from raw seafood: isolation and molecular identification of bacteria from the gut of Portunus pelagicus

Abstract

Portunus pelagicus is a marine commodity that is in great demand by consumers, especially coastal communities. Portunus pelagicus habitat in the sea allows contamination from microorganisms and marine waste. Proper cleaning and processing of Portunus pelagicus can cause foodborne disease. One part of Portunus pelagicus that contains a lot of contaminants is the gut. The process of isolation and molecular identification of bacteria from the gut of Portunus pelagicus is important. Portunus pelagicus samples that have been taken from the gut and extracted. Portunus pelagicus gut extract was put in NA media and continued into BAP media. Pure bacterial cultures were isolated using CIAA phenol DNA method and amplified using 16S rRNA followed by sequencing. Four bacterial isolates were obtained from the gut of Portunus pelagicus, namely PorTRJ6, PorTRJ8, PorTRJ9, PorTRJ10. PorTRJ6 are β-hemolytic bacteria and PorTRJ8, PorTRJ9, PorTRJ10 are α-hemolytic bacteria. Based on the results of sequencing the bacterial isolates had similarity with Vibrio parahaemolyticus, Uncultured bacterium clone RS-E27, Staphylococcus haemolyticus, Staphylococcus sp. Some bacteria found in the gut of Portunus pelagicus can cause foodborne disease. Portunus pelagicus is a high-protein seafood that can be toxic if it is not processed cleanly and properly.