The thermostabilizing domain, XynA, of Caldibacillus cellulovorans xylanase is a xylan binding domain

Abstract

We show that the N-terminal ‘thermostabilizing domain’ (TSD) of the xylanase, XynA, from the thermophilic bacterium Caldibacillus cellulovorans also acts as a xylan binding domain. Affinity electrophoresis experiments show that this TSD selectively binds soluble xylan and binds weakly to hydroxyethylcellulose. Based on this, and previously reported evidence, we propose that xylanase-associated TSDs are xylan binding domains.