The Thermochemotherapy Effect of Mn<sub>0.5</sub>Zn<sub>0.5</sub>Fe<sub>2</sub>O<sub>4</sub> Magnetic Fluid Hyperthermia Combined with As<sub>2</sub>O<sub>3</sub> on GLC-82 Cells

Abstract

AIM: To study the preparation and characterization of the Mn0.5Zn0.5Fe2O4 (MZF) magnetic nanoparticles and the antitumor function of MZF nanomagnetic fluid hyperthermia (NMFH) combined with As2O3 on GLC-82 cells.METHODS: The MZF magnetic nanoparticles were prepared by the method of chemical coprecipitation.,which were detected by transmission electron microscopy (TEM) and energy dispersive spectrometry (EDS). In vitro heating test of MZF magnetic fluid in an alternating magnetic field (AMF) was carried out. After that, we studied the therapeutic effects on cultured GLC-82 cells treated by thermochemotherapy of NMFH combined with As2O3 at various concentrations (2,5,8μM) were evaluated by MTT assay and flow cytometry assay (FCM).RESULTS: The MZF magnetic nanoparticles were prepared successfully. Their shapes are approximately global and their diameters are about 30 nm. EDS showed MZF magnetic nanoparticle contained Mn, Zn, Fe elements. The corresponding fluid of MZF magnetic nanoparticle could rise to 40~50°C due to different concentration in a AMF and keep stable from then on. MTT assay and FCM revealed that the thermochemotherapy effect of the MZF magnetic nanoparticles combined with As2O3 can cause significantly inhibit cellular growth observed by MTT test assay and obviously induce cellular apoptosis by FCM detecting (p<0.05). The apoptotic indexes of GLC-82 cells of the pure As2O3 group,pure MZF magnetic nanoparticles hyperthermia group and thermochemotherapy of NMFH combined with As2O3 group were 4.98%,16.30%,22.53%,respectively,while the apoptotic index of the negative control group was 0.56%.CONCLUSION: The MZF magnetic nanoparticles successfully were prepared through improved co-precipitation process and have good power absorption capabilities in the high frequency AMF, then they can rise to a steady temperature. MZF magnetic nanoparticles have hyperthermia effects on tumor and their antitumor effect significantly increase when combined with As2O3.