The therapeutic effect and mechanism of interleukin-10 on cerebral vasospasm caused by subarachnoid hemorrhage

Abstract

Objective To study the effect of interleukin-10(IL-10)on cerebral vasospasm following subarachnoid hemorrhage(SAH) in rabbit and explore the mechanism. Methods Thirty Japanese rabbits were randomly divided into Sham operation group(A), SAH group(B), SAH+ IL-10 group(C), SAH+ Zinc protoporphyrin(ZnPP)+ IL-10 group(D) and SAH+ ZnPP group (E).The animal model of cerebral vasospasmfollowingsubarachnoid hemorrhage was made by infusing autologous artery blood twice into the cisterna magna.The C, D, E group were respectively received IL-10, ZnPP through intraperitoneal injection after the SAH model was established. The tumor necrosis factor(TNF)-αand IL-6 levels in serum were detected by enzyme linked immunosorbent assay(ELISA) method 5 days postoperatively.The diameter and expression of heme oxygenase-1(HO-1) protein in basilar artery were observed. Results The animal model of cerebral vasospasm after subarachnoid hemorrhage was success.The basilar artery diameter of SAH groups was significantly shorter than the sham operation group(P< 0. 05)and the TNF-αand IL-6 levels were higher in SAH group(P< 0. 05), The diameter of group C and D[(733. 94±17. 28), (646. 11±9. 79)μm]was increased than group B and E[(595. 64±10. 15),(532. 81±17. 09) μm], the TNF-αand IL-6 levels in group C[(26. 27±1. 64),(58. 15±1. 38) ng/L] and D[ (43. 45±1. 77),(77. 17±1. 09) ng/L]were lower than group B[(53. 56±1. 27),(115. 93±1. 47) ng/L]and E[(60. 56±1. 79),(136. 45±1. 73) ng/L].There wasn't the expression of protein HO-1 in sham operation group and the content of HO-1 in C group(0. 446±0. 019) was higher than group B, D, E(0. 314±0. 014, 0. 251±0. 018, 0. 160±0. 011). Conclusion IL-10 can alleviate the cerebral vasospasm caused by subarachnoid hemorrhage and may be mediated by the HO-1 protein. Key words: Subarachnoid hemorrhage; Cerebral vasospasm; Interleukin-10; Heme oxygenase-1