Abstract
A detailed study was made of the temperature dependence of the intensities of certain bands in the Raman spectra of double-stranded calf thymus DNA. The temperature dependent Raman bands were assigned to structural components of the macromolecule by comparison with the behavior of temperature dependent intensities of simple model polymers and nucleotides. In addition to changes occurring near the melting temperature, complex changes were observed in Raman spectra at temperatures between 40 and 55°C, well below the melting temperature of this DNA. To determine the correlation between these Raman spectral changes and ultraviolet absorption changes in the pre-melting temperature range, we studied the temperature dependence of ultraviolet difference spectra for calf thymus DNA. Using a simple model we can account for the variety of Raman temperature dependences and qualitatively correlate these with the temperature dependences observed in the ultraviolet spectra. These new findings were obtained at a temperature range sufficiently low to be of significance for the study of DNA in vitro.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis
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