Abstract
RATIONALE: PTGDR gene has been associated with asthma susceptibility by several groups, including ours. In addition we have observed a stronger association of PTGDR with allergic asthma. In the present study we have evaluated the possible association of PTGDR gene with allergen sensitization.METHODS: In this study, 550 individuals (299 atopic patients and 251 non-atopic non-asthmatic controls). Skin prick tests with a battery of common aeroallergens were performed to both patients and controls. After PCR and direct sequencing haplotypic combinations of -613C>T, -549T>C, -441C>T, -197T>C and the non-previously described -95G>T SNPs of PTGDR were analyzed. Assessment of haplotypic interactions were performed with the Shesis software platform. EMQN guidelines were observed in all laboratory procedures.RESULTS: We found that the TCCTG (-613T, -549C, -441C, -197T and -95G) haplotype was more frequent in mite allergic patients (Fisher p=0.05), pollen allergic patients (Fisher p=0.026; OR:1.72[1.06-2.79]) and particularly in patients sensitized to both pollen and mites (Fisher p=0.005; OR:2.39[1.28-4.46] than in controls.CONCLUSIONS: The TCCTG haplotype was significantly associated with pollen and mite allergy. Interestingly, the CCT (-549C, -441C, -197T) haplotype has been previously described to be a high transcriptional efficiency haplotype which could determine a high PTGDR expression. In the present study we described an expanded combination of PTGDR SNPs associated with allergen sensitization. RATIONALE: PTGDR gene has been associated with asthma susceptibility by several groups, including ours. In addition we have observed a stronger association of PTGDR with allergic asthma. In the present study we have evaluated the possible association of PTGDR gene with allergen sensitization. METHODS: In this study, 550 individuals (299 atopic patients and 251 non-atopic non-asthmatic controls). Skin prick tests with a battery of common aeroallergens were performed to both patients and controls. After PCR and direct sequencing haplotypic combinations of -613C>T, -549T>C, -441C>T, -197T>C and the non-previously described -95G>T SNPs of PTGDR were analyzed. Assessment of haplotypic interactions were performed with the Shesis software platform. EMQN guidelines were observed in all laboratory procedures. RESULTS: We found that the TCCTG (-613T, -549C, -441C, -197T and -95G) haplotype was more frequent in mite allergic patients (Fisher p=0.05), pollen allergic patients (Fisher p=0.026; OR:1.72[1.06-2.79]) and particularly in patients sensitized to both pollen and mites (Fisher p=0.005; OR:2.39[1.28-4.46] than in controls. CONCLUSIONS: The TCCTG haplotype was significantly associated with pollen and mite allergy. Interestingly, the CCT (-549C, -441C, -197T) haplotype has been previously described to be a high transcriptional efficiency haplotype which could determine a high PTGDR expression. In the present study we described an expanded combination of PTGDR SNPs associated with allergen sensitization.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
