THE TARGET-CHEMOTHERAPY DIRECTED BY THE RADIATION

Abstract

The target-chemetherpy directed by the radiation was tested by the two methods; 1) facilitate the carboplatin; and 2) the capsules that is decomposed by irradiation IN VITRO. The human leukemic cell (OCI/M2) cultured in RPMI 1640 was treated by the 0.5 Gy of 60 Co γ ray after the 4μmol of carboplatin administration. The uptake of carboplatin expressed as Pt signal was measured by the micro PIXE camera. The increased uptake of carboplatin was observed in the cell. There was also an increased uptake of Cu . The chelating of Cu resulted in the maximum uptake of carboplatin under the combined treatment of radiation and carboplatin. As for the capsules, it was prepared by polymerizing the alginate by CaCl 2, whose core was carboplatin. The 0.5 Gy irradiation was performed to the capsules. The carboplatin's emission was observed using a micro PIXE camera. As a result, the carboplatin was emitted from capsules, which was almost 10 %. Those two methods (increased uptake of carboplatin by low dose irradiation and the capsules decomposed by irradiation) is considered to be useful for the better therapeutic gain and less adverse effect.