THE T344C-CYP11B2 POLYMORPHISM AS A RISK FACTOR FOR PREECLAMPSIA IN ROMANIAN PREGNANT WOMEN. ASSOCIATION WITH M235T (AGT) AND I/D (ACE) POLYMORPHISMS: PP.24.487

Abstract

Aim: 1. To determine the implication of the T344C polymorphism in the severity and the adverse outcome of preeclampsia-PE (gestational age at delivery, birth weight) 2. To evaluate the association between the T344C polymorphism and the M235T or I/D- ACE polymorphisms. Materials and Methods: We performed T344C, M235T and ACE genotyping using PCR-RFLP methods in 58 women with different types of preeclampsia and 59 normal pregnant women. Results: The risk for PE was 1.29 (95%CI [0.59- 2.79], p = 0.53) and 3.21 (95%CI [0.96- 10.78], p = 0.05) in women with the CT344 and CC344, respectively genotype. The risk for mild preeclampsia and severe preeclampsia was 1.49 (95%CI [0.63- 3.51], p = 0.24) and 2.01 (95%CI [0.55- 7.39], p = 0.32), respectively. 21 of 24 (87.5%) pregnant women who delivered at less than 36 weeks of gestation and were positive for the C344 allele had PE compared to 11 of 30 (36.66%) women who delivered at more than 36 weeks of gestation and were positive for the C344 allele, who had PE (OR 12.09, 95%CI [2.92- 49.99], p < 0.01). 14 of 73 (19.18%) women who delivered a baby weighing more than 2500 grams and were positive for the C344 allele had PE. All pregnant women who delivered a baby weighing less than 2500 grams and were positive for the C344 allele had PE. In the group positive for the M235T or I/D polymorphisms, a higher frequency of the T344C polymorphism was found in women with PE compared to normal pregnant women (57.5% vs. 35.48%, OR 2.45, 95%CI [0.93- 6.47], p = 0.05 and 59.62% vs. 46.51%, OR 1.7, 95%CI [0.75- 3.84], p = 0.2, respectively). Conclusions: The T344C polymorphism influences the severity of preeclampsia and could affect the adverse pregnancy outcome in preeclampsia. A higher risk of preeclampsia was found in women positive for both T344C and M235T polymorphisms.