Abstract
Extra-embryonic mesoderm (ExM), the earliest cells that traverse the primitive streak (PS), give rise to the endothelium as well as hematopoietic progenitors in the developing yolk-sac (YS). How a specific subset of ExM becomes committed to a hematopoietic fate remains unclear. Here we demonstrate using an embryonic stem cell (ESC) model that transient expression of the T-box transcription factor Eomesodermin (Eomes) governs hemogenic competency of ExM. Eomes regulates the accessibility of enhancers that SCL normally utilizes to specify primitive erythrocytes and is essential for the normal development of Runx1+ hemogenic endothelium. Single-cell-RNA-seq suggests that Eomes loss-of-function profoundly blocks formation of blood progenitors but not specification of Flk-1+ hematoendothelial progenitors. Our findings place Eomes at the top of the transcriptional hierarchy regulating early blood formation and suggest that hemogenic competence is endowed earlier during embryonic development than previously appreciated.
Accepted Version
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
